Characterization of Non-tuberculous Mycobacteria by Line Probe Assay Isolated from Pulmonary Tuberculosis Suspected Patients at Rajasthan

Non-tuberculous Mycobacteria (NTM) are emerging as an important opportunistic pathogen. Since clinical presentation of NTM infection is similar to tuberculosis (TB), patients present suspected TB or drug resistant TB. Presently in the National Tuberculosis Elimination Programme (NTEP ) not being speciated, but there urgent need characterize so that appropriate treatment can be given many species multi-drug resistant. The purpose study was use Line Probe Assay (LPA) i.e. GenoType Mycobacterium CM/AS assay for rapid early reporting and know pattern at Rajasthan. Sputum samples from 5000 Multi Drug Resistant (MDRTB) suspect were processed cultured on Growth Indicator Tube (MGIT). Culture isolates found positive mycobacteria Ziehl Neelsen (ZN) staining negative by MPT64 antigen test then subjected Among sputum 1520 (30.4%) patient mycobacteria, among these 1488 (97.9%) (MTB) 32 (2.1%) NTM, them 56.2% intracellulare, 21.8% abscessus, 9.3% fortuitum, 1% simiae showed invalid results. Incidence very low M. intracellulare abscessus most commonly isolated species, easy, reliable giving valid results 91% cases 3-5 days getting growth.